915
edits
Changes
no edit summary
* Amyloglucosidase 0.3 mg/mL
* Glucose quantification kit (Wako Autokit Glucose Buffer Solution cat # 439-9091; for protocol see http://www.wakodiagnostics.com/pi/pi_autokit_glucose.pdf)
* Glucose standard solution (200 or 500 mg/dL; Wako)
==Protocol==
# Quantify glucose using kit:
## Add 700 uL Glucose Buffer Solution with Color Reagent to a plastic cuvette.
## Add 1-5 uL glucose standard (200mg500mg/dL) for standard curve
## Add 10 uL digested glycogen
## Mix and incubate at 37C for 5 min
