Changes

RNA-seq Pipeline for Known Transcripts

244 bytes added, 20:12, 3 October 2011

added information about using FastQC

==Sequence Quality and Trimming==

# Run FASTQC to assess quality of reads from sequencer and:

# FASTQC available at http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/

## Open run_fastqc on a windows machine. Individually open each sequence file and allow it to analyse. Save this report.

## Check this report to decide if sequences need to be trimmed or discarded.

===Filter Sequences Using FastX-Toolkit===

# Filter for quality, if applicable

# Trim, if applicable

~~# FASTQC available at http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/~~

==Generate a Reference Genome==

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