915
edits
Changes
imported protocol
==Prepare the Cell (Not Auto-Fill)==
#Invert cell on black stand without base piece. Remove piston to fill line
#Pour in sample, replace base and place back on the press. Ensure valves are tightened
==Loading Sample==
#With the back valve open and the bottom valve closed insert tubing into sample
#Set French Press to Down and start. Suction caused by the piston moving out of the cell will bring sample into the cell
#When the base is at the bottom close the intake valve and stop the Press
==Lysing Sample==
#For bacteria 15 000 psi of cell pressure is sufficient. For a 1cm cell this corresponds to about 1100 psi on the gauge (see chart)
#Turn black knob all the way to the left
#Set Press to High and ensure both valves are securely closed
#Start the press. The cell will move until it encounters pressure and will stop moving (the motor will still be running)
#Slowly turn the knob until the gauge pressure reads desired pressure
#Slowly open valve until sample comes out, keep an eye on the pressure and make sure it doesn’t drop. Continue until piston is fully depressed. Close bottom valve
#Reload sample if necessary
==Cleaning/Storage==
#Disassemble and rinse all parts with 70% ethanol then distilled water
#Normally cells are stored at 4C to reduce sample heating
#Invert cell on black stand without base piece. Remove piston to fill line
#Pour in sample, replace base and place back on the press. Ensure valves are tightened
==Loading Sample==
#With the back valve open and the bottom valve closed insert tubing into sample
#Set French Press to Down and start. Suction caused by the piston moving out of the cell will bring sample into the cell
#When the base is at the bottom close the intake valve and stop the Press
==Lysing Sample==
#For bacteria 15 000 psi of cell pressure is sufficient. For a 1cm cell this corresponds to about 1100 psi on the gauge (see chart)
#Turn black knob all the way to the left
#Set Press to High and ensure both valves are securely closed
#Start the press. The cell will move until it encounters pressure and will stop moving (the motor will still be running)
#Slowly turn the knob until the gauge pressure reads desired pressure
#Slowly open valve until sample comes out, keep an eye on the pressure and make sure it doesn’t drop. Continue until piston is fully depressed. Close bottom valve
#Reload sample if necessary
==Cleaning/Storage==
#Disassemble and rinse all parts with 70% ethanol then distilled water
#Normally cells are stored at 4C to reduce sample heating