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GoTaq PCR Genotyping

165 bytes added, 14:50, 6 December 2010
updated protocol and added categories
==Materials==
*GoTaq Master Mix
*Primer Mix (from 100uM Primer Stocks): Add 10uL of forward and reverse primer, 20uL total, into 980uL of dH20 to make a 10uM 1uM solution. *Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 14uL Nuclease-Free Water, and 1uL 15uL of template . If doing several samples make a master mix of primer mix + template (25 uL GOTaq + 10 uL Primer Mix)x # of Samples + 1
*Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr.
*Add this mixture to each PCR tube.
*Label and add each template to the corresponding PCR tube.
*Run PCR under genotyping>inoki (~1 hour) appropriate [[Genotyping Program]] *Load samples in gel and run on 30V 130V (horizontally.) [[Category:Mouse Work]][[Category:PCR]][[Category:Genotyping]]