915
edits
Changes
clarified some points
# Incubate 15 min at 37C with occasional shaking
# Place on ice for 15 min.
# Spot 90 uL of reaction mixture onto GF/A filters. Wait 3s and immerse # Place in a well of a 6 well dish.# Add ~2mL of ice cold 70% ethanol at 4C.
# Wash 15 min at 4C
# Wash twice 10-20 min at room temperature with fresh 70% ethanol.
# Air dry overnight on a paper towel and count in 5 mL scintillation fluid. Also count 5 uL of both the - and + G6P solution to calculate total incorporation