915
edits
Changes
initial page
==Materials==
*S2 Cells. See [[Culturing S2 Cells]]
*dsRNA. See [[Preparation of dsRNA]]
*S2 Cell Media. See [[Culturing of S2 Cells]]
==Protocol==
*Take a near-confluent plate of S2 cells aspirate media and scrape into 1 mL of fresh media.
*Add 9 mL of fresh media and pipet to mix.
*Using hemocytometer count cells.
**Add ~100 uL cells under coverslip to hemocytometer.
**Count one row of 4 squares. For cells touching the edges of the square, only count the top and left line to avoid counting the same cells twice.
**Record the number of cells (should be 30-150 cells per row) and Calculate cell concentration, where \alpha is number of cells per row
<math>\alpha\times 4 (rows per square) \times 10^5 (dilution factor)</math>
[[Category:Cell Culture]]
[[Category:Knockdown]]
[[Category:Drosophila]]
*S2 Cells. See [[Culturing S2 Cells]]
*dsRNA. See [[Preparation of dsRNA]]
*S2 Cell Media. See [[Culturing of S2 Cells]]
==Protocol==
*Take a near-confluent plate of S2 cells aspirate media and scrape into 1 mL of fresh media.
*Add 9 mL of fresh media and pipet to mix.
*Using hemocytometer count cells.
**Add ~100 uL cells under coverslip to hemocytometer.
**Count one row of 4 squares. For cells touching the edges of the square, only count the top and left line to avoid counting the same cells twice.
**Record the number of cells (should be 30-150 cells per row) and Calculate cell concentration, where \alpha is number of cells per row
<math>\alpha\times 4 (rows per square) \times 10^5 (dilution factor)</math>
[[Category:Cell Culture]]
[[Category:Knockdown]]
[[Category:Drosophila]]