Changes

Collecting and Storing Mouse Serum

356 bytes added, 18 January
created serum page
==Materials==
*Heparinzed capilaries (Fisher cat# 22-362574)

==Protocol==
#Collect about 2/3 of a capillary of blood, typically via a retro-orbital bleed.
#Transfer blood to labelled eppendorf tubes.
#Let blood clot for 30 min on ice.
#Centrifuge 20 min at 2000g (about 5000 RPM in eppendorf 5415C centrifuge).
#Gentry remove liquid layer and store at -80