Changes

*Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip.*Dissolve rapamycin at 20 mg/mLin water.*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).