Changes

Surface Plasmon Resonance - Protein Lipid Interactions

4 bytes removed, 14:06, 10 June 2009

→‎Preparation of Liposomes

==Preparation of Liposomes==

#Combine DOPC + C16 lipid (or DOPC alone) at a ratio of 97:3 and dry under nitrogen (~~150uL~~ 15uL PC or ~~145~~14.5uL PC + 40.5 uL PI)

#Resuspend to 10 mM total lipid with HBS-N (100 uL). Vortex thoroughly and sonicate in water bath

#Correct pH to 7.4 using pH paper and 0.6uL aliquots of 1M NaOH

*Tightly attach syringe and place on blcok.

*Pass 250uL of milliQ water through the filger ~5 times.

*Discard the water and inject the sample through the filter 10 times.

==Preparation of Surface==

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