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*Prepare a 1 g/10mL solution of glucose in the unlikely case that some animals become hyperketonemic.
*Weigh/MRI mice ahead of time, mark tails if necessary with different colors for rapid identification and take fasting ketone measurement via a tail clip.
*Prepare bOHB syringes with 1.5 g/KG mouse weight (ie for a 30g mousewith 15g lean body mass, 300 uLuse 15uL).
*At approximately 1 min intervals, inject appropriate amount of bOHB into intraperitoneal cavity of the mouse.
**Immobilize mouse and restrain tail with one hand.
*Analyze data by both % change from fasting ketones and absolute values. Our preferred outcome is to report fasting ketone levels and report percent change as a figure.
*The preferred statistical model is a mixed linear model using the time points as ordinate values and testing for a main effect or an interaction of the treatment/genotype. Use the R lme4 package for this.
[[Category:Ketogenesis]]