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Real Time PCR From Cell Culture

28 bytes removed, 20:12, 26 May 2009
→‎Plate Preparation
===Plate Preparation===
#Book 3h on qPCR machine at http://felix2.lsi.umich.edu/ORS/index.php?UID=2fd952b00de25
#Prepare primer mix from 5 uM primer pair stocks by adding 0.4 uL primers + 4.6 uL water per reaction
#Get 96 well block and keep on rack. Do not touch bottom of plate.