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Real Time PCR From Cell Culture

68 bytes added, 20:11, 26 May 2009
→‎Materials
*SyberGreen PCR Master Mix Applied Biosystems
*96 well qPCR plate
*Primers (Dilute to 0.22 5 uM mixture of fwd and revand then make a working solution of 0.4 uL + 4.6 uL water per reaction)
*Generate primers using http://pga.mgh.harvard.edu/primerbank/index.html
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Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/Special:MobileDiff/129"

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