QPCR: Difference between revisions

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 ===Run Protocol===
-#Open LightCycler software.
+* Set up run using [[Set up qPCR Run on Thermo Cloud and QuantStudio|Thermo Cloud/QuantStudio]] or  [[Set up qPCR Run on Roche LightCycler|Roche LightCycler]]
-#Click new experiment.
-#Choose SYBR Green option from drop down and make sure that the correct block is in place (it will say 96 or 384).
-#Click apply template.
-#Go to templates>run templates> bridges SYBR Green 384.
-#Load plate and click 'start run'.
-#This protocol is set to do the following:
-#Activate- 1 cycle @95 degrees for 10s.
-#Amplify- 45 cycles @95, 60 and 73 degrees for 15s, 15s and 10s, respectively.
-#Melt- 1 cycle @95, 40, 65, 95 and 40 degrees for 1m, 1m, 1s, continuous and 10s, respectively.
 ==Calculations==