Insulin Tolerance Test: Difference between revisions
wrote section regarding fat mass considerations |
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*Scale | *Scale | ||
*Beaker for weighing mice | *Beaker for weighing mice | ||
*echoMRI if the mice differ in body fat levels (see [[ | *echoMRI if the mice differ in body fat levels (see [[#Taking_into_Account_Differences_in _Fat_Mass]]) | ||
*Syringes | *Syringes | ||
*0.1 U/mL humulin in PBS (make as 10uL of 100 U/mL in 10 mL, sterile filtered). This will correspond to 1 U/kg injections. If you are using a higher or lower dose of insulin, add more or less to the 10 mL of PBS, so that injections are 10 uL/g of mass. | *0.1 U/mL humulin in PBS (make as 10uL of 100 U/mL in 10 mL, sterile filtered). This will correspond to 1 U/kg injections. If you are using a higher or lower dose of insulin, add more or less to the 10 mL of PBS, so that injections are 10 uL/g of mass. | ||