Phosphate Buffered Saline

Recipe

• Start with 400ml of distilled water:
• Add 4 g of NaCl.
• Add 0.72 g of KCl.
• Add 0.72 g of Na₂HPO₄.
• Add 0.12 g of KH₂PO₄.
• Adjust the pH to 7.4 with HCl.
• Add distilled water to a total volume of 500 mL.