MTM1 Genotyping

See http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153

Primers

MTM1 Fwd: GGC CCC ATT AAG GGA AGA GT
MTM1 WT Rev: GAT CTT CTG GGA AAG GCA CA
MTM1 Mut Rev: GCC AGA GGC CAC TTG TGT AG

PCR Program

94 for 2 min
10 cycles of:
- 94 for 20sec
- 65 for 15sec with -0.5 C per cycle
- 68 for 10sec
28 cycles of:
- 94 for 15sec
- 60 for 15sec
- 72 for 10sec
72 for 1 min
4 hold

Expected Results

File:MTM1 Genotyping Result.jpg

Last modified on 21 January 2013, at 15:17