Farnham Lysis Buffer

RIPA Buffer (for 50mL lysis buffer)

	Final Concentration	per 50 mL	Stock	Location
PIPES pH 8.0	5 mM	250 uL	1M	4C
KCl	85 mM	4.25 mL	1M	Solutions Shelf
NP-40	0.5%	0.357 mL	70%	Chemical Shelf
Protease Inhibitors		1 mini tablet/1X		4C