[[Category: Transfection]]
...
720 bytes (113 words) - 19:55, 9 March 2011
[[Category: Transfection]]
...
1 KB (155 words) - 21:00, 11 July 2012
[[Category:Transfection]]
...
2 KB (274 words) - 15:02, 14 December 2009
==Transfection Procedure==
* The morning of the transfection day, replace the media with fresh DMEM '''without PSG''' and containing 10
...
1 KB (199 words) - 16:15, 19 December 2017
[[Category: Transfection]]
...
1 KB (196 words) - 16:57, 22 February 2013
[[ Category: Transfection ]]
...
1 KB (198 words) - 13:22, 29 August 2011
==Transfection Procedure==
* The morning of the transfection day, replace the media with fresh DMEM '''without PSG''' and containing 10
...
1 KB (199 words) - 16:15, 19 December 2017
...[[Fugene Transfection of 293T/COS Cells|Fugene]], [[Lipofectamine Plasmid Transfection|Lipofectamine]] or other protocol
...
548 bytes (74 words) - 14:18, 16 September 2010
* The day before the transfection, seed 3.8 x 10<sup>6</sup> cells into a fresh plate. Seed cells into media
==Transfection Procedure==
...
2 KB (329 words) - 16:20, 8 December 2017
...DNA:PEI ratio and checking the number of fluorescent cells 1-2 days after transfection. Follow the protocol in [[Production of LentiCRISPR Viruses]] starting aft
[[ Category: Transfection ]]
...
888 bytes (130 words) - 16:16, 8 December 2017
[[ Category: Transfection ]]
...
743 bytes (95 words) - 20:02, 7 April 2020
[[Category: Transfection]]
...
720 bytes (113 words) - 19:55, 9 March 2011
[[Category: Transfection]]
...
1 KB (155 words) - 21:00, 11 July 2012
[[Category: Transfection]]
...
1 KB (196 words) - 16:57, 22 February 2013
[[ Category: Transfection ]]
...
1 KB (198 words) - 13:22, 29 August 2011
===Transfection of Cells===
...
1 KB (185 words) - 19:59, 20 September 2012
*[[Fugene Transfection of 293T/COS Cells]]
*[[Lipofectamine Plasmid Transfection]]
...
4 KB (484 words) - 12:10, 15 August 2016
[[Category:Transfection]]
...
2 KB (274 words) - 15:02, 14 December 2009
#Infect 293A cells by adding 40–50% of the primary transfection viral supernatants (i.e., 0.5–1.0 ml of the 2.0 ml viral lysate) to each 25
...
6 KB (872 words) - 20:56, 11 July 2012