...e assembled contig to check for errors. Ignore errors only at ends of one sequencing run.
[[Category: Sequencing]]
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846 bytes (134 words) - 15:31, 20 April 2011
...etails. Align those clones with the empty vector full sequence or the hU6 sequencing of the empty vector, available at http://www.addgene.org/42335/sequences/.
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2 KB (324 words) - 19:41, 19 October 2017
#Send for sequencing, preferably with primers reading both forward and reverse of the mutation
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427 bytes (73 words) - 13:24, 27 May 2009
*Typically we will select Sanger Sequencing (the only low throughput option)
**Size of the insert you are sequencing
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2 KB (329 words) - 16:20, 24 October 2017
# Select '''Enter Individual DNA Sequencing Requests'''
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646 bytes (108 words) - 18:34, 5 March 2014
#Sequence positive clones with pAdtrack sequencing primer.
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2 KB (353 words) - 19:47, 13 December 2010
[[Category: Next Generation Sequencing]]
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5 KB (760 words) - 16:08, 8 November 2011