| The Role of G proteins in insulin action
| The Role of G proteins in insulin action
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684 bytes (89 words) - 17:50, 3 January 2012
...ne bound proteins) in PBS or ice cold 10% methanol (for cytoskeleton bound proteins)
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This method is most accurate with highly purified proteins.
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*Add 1.5mL of 1x HNG to GST and GST-EEA1 proteins. (-80°C)
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940 bytes (146 words) - 17:48, 31 July 2012
...ernight at 4oC with 5mg/ml the bacterial expressed and purified GST-fusion proteins in 0.5% fatty-acid free BSA (Sigma) in TBS with 0.1% Tween20.
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** Database: Reference Proteins (refseq_protein)
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was performed using standard protocols. Proteins were blotted from a 6% gel and a 4-20% gel
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[[ Category: Proteins ]]
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2 KB (355 words) - 17:46, 31 July 2012
[[ Category: Proteins ]]
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3 KB (572 words) - 17:28, 31 July 2012
*[[Purification of GST Fusion Proteins]]
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4 KB (484 words) - 12:10, 15 August 2016
...aldehyde, and amino-acids in alkaline media and is also used to dissociate proteins.
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7 KB (1,078 words) - 19:17, 13 September 2019
* This step serves to “preclear” the chromatin, i.e., to remove proteins or DNA that may bind nonspecifically to the Protein G agarose.
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11 KB (1,742 words) - 15:23, 10 May 2018