*Mouse Tissues (Frozen)
#Add a stainless steel bead and keep tissues on ice. Homogenize using Qiagen Tissue Lyser (3 min at 25Hz for WAT/Liver
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1 KB (253 words) - 16:06, 25 April 2019
*Place mouse in isofluorane chamber and lightly anesthetize the animal.
*Remove mouse from chamber
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4 KB (624 words) - 17:05, 8 June 2020
*[[Triglyceride Assay from Cells and Tissues]]
===Mouse/Fly Protocols===
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4 KB (484 words) - 12:10, 15 August 2016
[[Category:Mouse Work]]
[[Category:Tissues]]
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882 bytes (140 words) - 21:43, 5 January 2018
[[Category:Mouse Tissues]]
[[Category:Mouse Work]]
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2 KB (376 words) - 13:02, 19 June 2017
...y an intraperitoneal injection of insulin. As glucose is transported into tissues and glucose production is suppressed, glucose levels drop. A more insulin
...he insulin sensitivity of the mice, and this is based on a normal C57BL/6J mouse on chow.
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4 KB (725 words) - 16:43, 10 February 2025
[[ Category: Mouse Tissues ]]
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1 KB (173 words) - 14:28, 2 August 2017
...on as you normally would for RNA extraction, ie by mechanically disrupting tissues in Qialyser. Approximately 20 mg of tissue is sufficient.
[[ Category: Mouse Tissues ]]
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3 KB (417 words) - 17:42, 25 September 2015
*Mouse Tissue (50-100 mg, about a 3mm cube)
[[Category:Mouse Work]]
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3 KB (478 words) - 20:51, 22 October 2018