*Br-POPC (Avanti Polar Lipids Cat# 850418P). Dissolve 10 mg in 1.08 mL of 1:1 CHCl3:MeOH with 0.1% HCl f
*Phosphoinositide of interest. (Avanti Polar Lipids Dioleyl tail groups). Dissolve 0.5 mg in ~500 uL (depending on Molecular W
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2 KB (260 words) - 13:01, 2 November 2010
#Extract Lipids:
[[Category: Inositol Lipids]]
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2 KB (238 words) - 15:44, 7 August 2009
* I used C8 lipids and dissolved to 1 mM in PBS and frozen in small aliquots at -80.
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720 bytes (113 words) - 19:55, 9 March 2011
[[ Category: Lipids ]]
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1 KB (171 words) - 16:44, 10 February 2025
...is loaded on each surface. Start with lane 4 and move to lane 1-PC only, lipids can migrate accross wells over time, so load in a way to minimize contamina
##Repeat previous step for all lipids.
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5 KB (753 words) - 14:40, 14 April 2011
...are ready to be viewed. • View the plates on a phase contrast microscope. Lipids will appear red and the nuclei will appear blue.
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3 KB (539 words) - 15:30, 20 April 2015
[[Category: Inositol Lipids]]
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8 KB (1,193 words) - 13:28, 21 June 2010