...u believe your protein is bound to (for ChIP-seq peaks refer to [[Locating ChIP-seq peaks from ENCODE]]. Make sure this the genetic sequence is species app
...ndicate on the primer database and in the name on the label that these are ChIP primers so as not to be confused with normal qPCR primers.
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# Scroll to ChIP-seq and click “view matrix”
...for genes you believe your protein is bound to [[RT-PCR primer design for ChIP]]
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[[ Category: ChIP ]]
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ChIP Elution Buffer (make fresh)
# Using the Sonics VibraCell Sonicator, sonicate each 1.0 ml ChIP sample on ice, in a cold room, at Power Output 5 watts 6 times for 30 secon
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6 KB (1,077 words) - 21:43, 10 June 2016
[[ Category: ChIP ]]
...t.org/documents/6ecd8240-a351-479b-9de6-f09ca3702ac3/@@download/attachment/ChIP-seq_Protocol_v011014.pdf here]. The original citation for this methodology
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[[ Category: ChIP ]]
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* CM5 Biacore Chip
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# Input: expression matrix, chip environments
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*L1 Sensor Chip (Biacore)
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5 KB (753 words) - 14:40, 14 April 2011