Insulin Tolerance Test: Difference between revisions
added ordering details for humulin |
m fixed glucose typo |
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*Typically starve the mice at 9AM and aim to start injections at 3 PM | *Typically starve the mice at 9AM and aim to start injections at 3 PM | ||
*Weigh mice, mark tails if necessary and take fasting glucose measurement via a tail clip. | *Weigh mice, mark tails if necessary and take fasting glucose measurement via a tail clip. | ||
*Prepare | *Prepare insulin syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||
*At approximately 1 min intervals, inject appropriate amount of insulin into interperitoneal cavity of the mouse. | *At approximately 1 min intervals, inject appropriate amount of insulin into interperitoneal cavity of the mouse. | ||
**Immobilize mouse and restrain tail with one hand | **Immobilize mouse and restrain tail with one hand | ||