Rapamycin Injections: Difference between revisions
added dosage volume |
added rapamycin treatment from Lamming et al |
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*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week). | *Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week). | ||
*Inject 10 uL/g body weight | *Inject 10 uL/g body weight | ||
==Alternate Rapamycin Injection Regimen== | |||
* Phenotypes seem to be different with rapamycin as treated by Lamming et al. | |||
* This protocol is from Lamming et al http://dx.doi.org/10.1126/science.1215135 | |||
<blockquote> | |||
rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally | |||
once daily with either 2 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a | |||
concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days. | |||
</blockquote> | |||