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Created page with 'PBND Solution: Tail Lysis Buffer -50mM KCl -10mM Tris HCl (pH~8.3) -0.1 mg/mL MgCl2, 6H20 -0.1 mg/mL Gelatin -0.45% (NP-40) Jgepal -0.45% Tween 20 Protocol 1.) Combine 200uL o...'
PBND Solution: Tail Lysis Buffer
-50mM KCl
-10mM Tris HCl (pH~8.3)
-0.1 mg/mL MgCl2, 6H20
-0.1 mg/mL Gelatin
-0.45% (NP-40) Jgepal
-0.45% Tween 20
Protocol
1.) Combine 200uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf tube
2.) Incubate at 55 degrees (3hrs-O/N)
3.) Incubate at 85 degrees for 45 min
4.) PCR
-50mM KCl
-10mM Tris HCl (pH~8.3)
-0.1 mg/mL MgCl2, 6H20
-0.1 mg/mL Gelatin
-0.45% (NP-40) Jgepal
-0.45% Tween 20
Protocol
1.) Combine 200uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf tube
2.) Incubate at 55 degrees (3hrs-O/N)
3.) Incubate at 85 degrees for 45 min
4.) PCR