Changes

==PBND Solution: Tail Lysis Buffer==-* 50mM KCl-* 10mM Tris HCl (pH~8.3)-* 0.1 mg/mL MgCl2, 6H20-* 0.1 mg/mL Gelatin-* 0.45% (NP-40) Jgepal -* 0.45% Tween 20

==Protocol==1.) # Combine 100-200uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf tubeor in a well of a 96 well PCR plage2.) # Incubate at 55 degrees (3hrs-O/N)3.) # Incubate at 85 degrees for 45 min 4.) # Run PCR