===Run Protocol===
#Open LightCycler software.#Click new experiment.#Choose SYBR Green option from drop down * Set up run using [[Set up qPCR Run on Thermo Cloud and make sure that the correct block is in place (it will say 96 QuantStudio|Thermo Cloud/QuantStudio]] or 384).#Click apply template.#Go to templates>run templates> bridges SYBR Green 384.#Load plate and click 'start run'.#This protocol is set to do the following:#Activate- 1 cycle @95 degrees for 10s.#Amplify- 45 cycles @95, 60 and 73 degrees for 15s, 15s and 10s, respectively.#Melt- 1 cycle @95, 40, 65, 95 and 40 degrees for 1m, 1m, 1s, continuous and 10s, respectively. [[Set up qPCR Run on Roche LightCycler|Roche LightCycler]]
==Calculations==