Sending Samples for DNA Sequencing
- Create an account or log in at http://128.169.4.12/
- Select Economy under Service Requested and enter the number of samples in the box above.
- Select Enter Individual DNA Sequencing Requests
- Enter the tube and select the primers (you will have to provide the primers if doing economy). Hit enter, print out the confirmation page for your notes.
- Write the template name on each of your tubes
- In each tube add:
- 1 uL of template (unless the plasmid DNA concentration is >500 ng/uL in which case dilute it to 50-300 ng/uL
- 3.2 uL of 1 uM Primer (Dilute the primer stock 100X)
- 7.8 uL of water
- Drop the samples off at the MRC.