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Materials
- Agarose
- TAE Buffer (1X): Dilute from 100X stock
- Thermo Apparatus (Mold, Holder, Comb and Gel Rig)
Prepare Gel (BioRad Gel Rig)
- Place plastic mold securely in holder
- Put well comb in mold
- Weight out 1.0g agarose and add 100 mL of TAE (1% solution) then return TAE to shelf
- Microwave 90s until dissolved
- Add 2 uL Gel Red. Swirl to mix and eliminate sediments
- Pour into mold
- Place gel in cold room if in a hurry
- After loading, run gel at 100V