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Cre PCR
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PCR Program
Cre (F) GCATT ACCGG GCAAC GAGTG ATGAG
Cre (R) GAGTG AACGA ACCTG GTCGA AATCA GTGCG
95C 1min
35 Cycles of:
95C 10s
55C 30s
72C 45s
72C 5min
4C forever