The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

PCR Program

  • Cre (F) GCATT ACCGG GCAAC GAGTG ATGAG
  • Cre (R) GAGTG AACGA ACCTG GTCGA AATCA GTGCG
  • 95C 1min
  • 35 Cycles of:
    • 95C 10s
    • 55C 30s
    • 72C 45s
  • 72C 5min
  • 4C forever