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ImageJ Download
- Download ImageJ from http://imagej.nih.gov/ij/download.html
- To to open ImageJ, right click on software in applications folder
- Since the program is downloaded from the internet it will not open up automatically and you must go through applications folder.
Adipocyte Counting Macro
Adipocyte Cell Counting
- With macro installed, click on segmentation: "s"
- Set boundaries for sizing of cell
- Use 40-40,000 for size typically.
- Change thresholding method to Default.
- Open image of interest.
- Click on segmentation "s"
- Select freehanding tool to outline any cells that were not counted
- To add freehanded cell to ROI manager, click command + T
- Once all cells have been outlined, and misnumbered/outlined cells have been removed, click on measure
- Copy and save measurements to Excel spreadsheet
To save image of outlined cells
- Make sure all cells are outlined.
- Click on "Flatten"
- Save flattened image to folder.