50X TAE Buffer

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Reagents

900mL ddH2O
242g Tris Base
57.1 mL Glacial Acetic Acid
18.6g EDTA

1. Add Tris Base, Glacial Acetic Acid, and EDTA to 500mL water in 1L Erlenmeyer Flask and mix well by swirling the flask. 2. Add remaining 400mL water and mix on stir plate (or my swirling).