Farnham Lysis Buffer
RIPA Buffer (for 50mL lysis buffer)
Final Concentration | per 50 mL | Stock | Location | |
---|---|---|---|---|
PIPES pH 8.0 | 5 mM | 250 uL | 1M | 4C |
KCl | 85 mM | 4.25 mL | 1M | Solutions Shelf |
NP-40 | 0.5% | 0.357 mL | 70% | Chemical Shelf |
Protease Inhibitors | 1 mini tablet/1X | 4C |