TaqMan SNP Assay

• TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).
• TaqMan® Genotyping Master Mix (supplied at 2X)(Applied Biosystems).
• StepOnePlus Real-Time PCR System (Applied Biosystems).
• PC/Laptop with StepOnePlus Software installed.
• MicroAmp Fast 96-Well Plates.
• Optical Plate Covers.
• Nuclease-Free Water
• Sample Genomic DNA (extracted as described in Blood DNA Extraction)
• Postive control(s) (Minor allele) (optional, but recommended)

1. Dilute gDNA samples to ng/ul.
2. Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
3. Make up Reaction Master mix (see table below).
4. Add Reaction Mix (5.25 ul) added to each well (see Table 2.)
5. Diluted gDNA samples each added to a single well (see Table 2.)

Table 1. Reaction Mix for full 96 well plate

Table 2. for full 96 well plate