Amitryptyline Treatment of Mice
Revision as of 16:04, 23 January 2014 by Davebridges (Talk | contribs) (wrote protocol for amitriptyline in water)
Revision as of 16:04, 23 January 2014 by Davebridges (Talk | contribs) (wrote protocol for amitriptyline in water)
We use Amitiptyline from Sigma (A8404).
For drinking water administration ~10 mg/kg/day
We have approval for 1-100 mg/kg/day. Still need to pilot these effects
- Makes 1L of drinking water
- dissolve 0.092g in 1L water
Calculation
- 10 mg drug/kg/day because mice drink ~5mL /day so want 2 mg Dex/kg/mL
- average mouse weighs 0.03kg so want 0.06 mg Dex/mL or 60 mg/L
- This assumes that the mice are about 30g and they drink 5mL/day. The volume drank per cage should be monitored.