TBST
Revision as of 14:52, 12 April 2013 by Davebridges (Talk | contribs) (copied recipe from saltiel lab buffers)
Revision as of 14:52, 12 April 2013 by Davebridges (Talk | contribs) (copied recipe from saltiel lab buffers)
10X TBS Buffer
To make 1X TBS, dilue 10X with water, and add 10 mL/10L Tween 20 (Sigma P7949-500ml)
Component | Weight per 10 L | Catalog Number |
---|---|---|
Tris Base | 606 g | Sigma T6066-5KG |
NaCl | 877 g | Sigma S7653-5KG |
HCl | 360 mL | Sigma 258148-2.5L |