Genotyping Program

Revision as of 16:44, 9 October 2012 by Davebridges (Talk | contribs) (added ob/ob genotyping)

Revision as of 16:44, 9 October 2012 by Davebridges (Talk | contribs) (added ob/ob genotyping)

Allele Program Fragment Size(s) Notes
TSC-floxed Inoki PCR Two bands if heterozygote
Cre Inoki PCR Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14 Inoki PCR Two bands if heterozygote
TC10b TC10b KO - 500bp, WT-1kb Two bands if heterozygote
ob/ob Genotyping ob/ob KO - 123bp, WT-104kb, control 191 bp Two reactions, see ob/ob Genotyping
CAP CAP Two bands if heterozygote
Vinexin Vinexin