Analysing Sequencing Results with SeqMan
- Open Seqman (under DNAStar Lasergene)
- Download all sequence files from FTP site to a working folder (ftp://seqcore.brcf.med.umich.edu/)
- In SeqMan go to Sequence -> Add...
- In your working folder select AB1 files and then Add then Done
- If adding a reference sequence click Add Sequence... button then enter the nucleotide accession number and save this to your working folder.
- Click Assemble button on the new window
- A contig should be assembled on the window to the right. Double click on this.
- Scan through the assembled contig to check for errors. Ignore errors only at ends of one sequencing run.
- To see the chromatogram click on the grey triangle at the left of the sequence
- Save the assembly as a SeqMan file.