CPY Assay

Carboxypeptidase Secretion Assay

• Revive Yeast Cells (2-3) days
• Pick one colony and make a 1/2 inch x 1/2 inch square patch of yeast. (Does this have to be selective plates? or are YPD plates always used?)
• Place nitrocellulose filters on gel and incubate overnight at 23(So you're not actually moving the patch to the filter, but just allowing it to touch?)
• Wash filter in water (by adding water to dish and rocking?)
• Primary antibody (polyclonal against cpy location?) how long?
• Secondary antibody (where/what is it and how long?)

Wash with pbs in between?

What is ECL?