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Bradford Assay

Revision as of 13:42, 23 April 2010 by Davebridges (Talk | contribs) (formatting change for PMID)

Revision as of 13:42, 23 April 2010 by Davebridges (Talk | contribs) (formatting change for PMID)

Materials

  • BioRad Protein Assay Dye Reagent Concentrate cat#500-0006
  • Disposable Plastic Cuvette

Protocol

  1. Dilute reagent 5X in water, stable for 2-3 weeks
  2. Pipet 1 mL into disposable plastic cuvette
  3. Add 1-10 uL of protein sample, cover with parafilm and mix
  4. Let sit 5-10 min to react
  5. Set spectrophotometer as follows:
    1. Go to protein assay then Bradford assay
    2. Set formula, then select more
    3. Set b=0.045 (or determine slope)
    4. Set dilution to be 1/vol (ie 0.1 for 10 uL)
    5. Blank then measure samples, absorbance must be less than 0.9
    6. Print (hit Recall, then enter, then print) and attach to experiment

Reference

Last modified on 15 March 2018, at 14:39