Bridges Lab Protocols

TORC1 Kinase Buffer

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mTORC1 Kinase Buffer (for 10mL lysis buffer)

Final Concentration per 10 mL Stock Location
HEPES, pH 7.4 25 mM 250 uL 1M 4C
KCl 50 mM 500 uL 1M Shelf
MgCl2 10 mM 100 uL 1M Shelf
ATP 250 uM 25 uL 100mM -20

see PMID 19200882

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