Designing and Preparing dsRNA

Revision as of 13:47, 3 August 2009 by Davebridges (Talk | contribs)

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Revision as of 13:47, 3 August 2009 by Davebridges (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Contents

Ordering dsRNA Primers
Preparing dsRNA

Ordering dsRNA Primers

If available, choose primers from Harvard DSRC site http://www.flyrnai.org/cgi-bin/RNAi_gene_lookup_public.pl
Add T7 RNA Polymerase sites to the 5' of each primer (TAATACGACTCACTATAGG)
Record primer and amplicon (DSRC)

Preparing dsRNA

see http://www.flyrnai.org/DRSC-PRS.html

Last edited 16 years ago by Davebridges