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Designing and Preparing dsRNA

Revision as of 13:27, 3 August 2009 by Davebridges (Talk | contribs) (intial page)

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Revision as of 13:27, 3 August 2009 by Davebridges (Talk | contribs) (intial page)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Contents

Ordering dsRNA Primers

  • If available choose primers from Harvard DSRC site http://www.flyrnai.org/
  • Add T7 RNA Polymerase sites to the 5' of each primer:

TAATACGACTCACTATAGG

Last edited 16 years ago by Davebridges