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Revision as of 13:48, 28 October 2023 by Sotu (Talk | contribs) (Created page with "== Cholesterol Assay == === Materials === * Reagent * Cholesterol Standard === Protocol === * Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL...")

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Revision as of 13:48, 28 October 2023 by Sotu (Talk | contribs) (Created page with "== Cholesterol Assay == === Materials === * Reagent * Cholesterol Standard === Protocol === * Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL...")

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Cholesterol Assay

Materials

  • Reagent
  • Cholesterol Standard

Protocol

  • Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL tube
  • Pipette 100ɥL of Reagent to 22 wells
  • Add 2-10ɥL of 10X stock dilution to wells in columns 2-6 in increments of 2ɥL. Each column will have 2 replicates per volume (1 replicate per row).
  • Repeat in columns 7-11 with 2-10ɥL of Cholesterol Standard (1X dilution)
  • Allow reaction to proceed for 15 minutes
  • Read absorbance
Last modified on 28 October 2023, at 13:50