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Buffer/CHAPS Lysis Buffer

Revision as of 15:08, 15 July 2009 by Davebridges (Talk | contribs) (wrote initial page)

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Revision as of 15:08, 15 July 2009 by Davebridges (Talk | contribs) (wrote initial page)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

CHAPS Lysis Buffer (for 10mL lysis buffer)

Final Concentration per 10 mL Stock Location
HEPES, pH 7.4 40 mM 400 uL 1M 4C
NaCl 120 mM 300 uL 4M Shelf
EDTA 2 mM 40 uL 0.5M Shelf
CHAPS 0.3% 300 uL 10%
NaPPi 10 mM 400 uL 0.25M Shelf
beta-glycerophosphate 10 mM 100 uL 1M 4C
NaF 50 mM 1mL 0.5M Shelf
Protease Inhibitors 1 mini tablet 4C
Last modified on 15 July 2009, at 15:26