Buffer/RIPA
Revision as of 13:33, 8 July 2009 by Davebridges (Talk | contribs) (mistake with NaF and NaPPi concentrations. Added note about NaVO3 location)
Revision as of 13:33, 8 July 2009 by Davebridges (Talk | contribs) (mistake with NaF and NaPPi concentrations. Added note about NaVO3 location)
RIPA Buffer (for 10mL lysis buffer)
| Final Concentration | per 10 mL | Stock | Location | |
|---|---|---|---|---|
| Tris pH7.4 | 50mM | 500uL | 1M | |
| Na Deoxycholate | 0.25% | 250uL | 10% | |
| NP-40 | 1% | 1mL | 10% | |
| NaCl | 150mM | 375uL | 4M | |
| EDTA | 1mM | 20uL | 0.5M | |
| NaVO3 | 1mM | 100uL | 1M | Biochemical Stocks (-20) |
| NaF | 5mM | 100uL | 500mM | |
| NaPPi | 25 mM | 1 mL | 250mM | |
| Protease Inhibitors | 1 mini tablet | 4C |