Open main menu

4EBP1/2 Genotyping

Revision as of 14:38, 8 June 2020 by Reddj (Talk | contribs)

Revision as of 14:38, 8 June 2020 by Reddj (Talk | contribs)

PRIMERS

  1. '4EBP1
  • 4EBP1- Fwd: GAT GGA GTG TCG GAA CTC ACC (1X)
  • 4EBP1- Rev: GAC CTG GAC AGG ACT CAC CGC (2X)
  • 4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X)
  1. 4EBP2
  • 4EBP2-Fwd: GGT GGG ACT GTC GGT CTT CTG (1X)
  • 4EBP2-Rev: CAG CAC CTG GTC ATA GCC GTG (2X)
  • 4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X)

Make primers at 1uM (Fwd1 + Rev1, Neo Fwd1/2 + Rev1, Fwd2 + Rev2, and Neo Fwd1/2 + Rev2). PCR 4EBP1 and 4EBP2 primers separately.

Last modified on 8 June 2020, at 14:41