Mc4r-Cone Genotyping
PRIMERS
- Fwd: CAC TCG GAG CTT CCC TGA CCC AG
- Rev: GAC CAT GGT TTC CGA CCC ATT
- Neo Rev: TTC CAA GCC TCT GAG CCC AGA
Make primers at 1uM (Fwd + Rev and Fwd + Neo Rev). PCR primers separately.
PCR PROTOCOL
PCR Step | Temperature | Time | Cycles | Notes |
---|