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Cre PCR

Revision as of 18:42, 25 April 2018 by Davebridges (Talk | contribs) (added primers)

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Revision as of 18:42, 25 April 2018 by Davebridges (Talk | contribs) (added primers)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Contents

PCR Program

  • Cre (F) GCATT ACCGG GCAAC GAGTG ATGAG
  • Cre (R) GAGTG AACGA ACCTG GTCGA AATCA GTGCG
  • 95C 1min
  • 35 Cycles of:
    • 95C 10s
    • 55C 30s
    • 72C 45s
  • 72C 5min
  • 4C forever
Last edited 7 years ago by Davebridges